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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: THF co-solvent pretreatment prevents lignin redeposition from interfering with enzymes yielding prolonged cellulase activity

Fig. 1

Comparison of glucose yields from enzymatic hydrolysis of solids prepared by i DSA and ii CELF pretreatments of switchgrass at cellulase loadings of 2–65 mg protein/g glucan in unpretreated switchgrass. Pretreatment reaction conditions were those that gave the highest total combined sugar yields at a loading of 65 mg protein/g enzyme, i.e., for DSA: 160 °C, 20 min, and 0.5 wt% sulfuric acid; for CELF: 150 °C, 25 min, and 0.5 wt% sulfuric acid at a 0.889:1 THF:water mass ratio

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