Fig. 3From: Targeting hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase for lignin modification in Brachypodium distachyonHCT transcript levels and lignin content and composition in stems of B. distachyon HCT-RNAi lines. (a–d) HCT transcript levels relative to housekeeping gene tubulin alpha-1 chain (Bradi1g10150) in T0 (a) and T1 (b) generation from single HCT1-RNAi lines, and T0 (c) and T1 (d) generation from double HCT1:HCT2-RNAi lines. e–h Lignin monomer composition determined by thioacidolysis in lower stem internodes of the same lines as shown in a–d. H, G, S = yields of the thioethylated products of p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) lignin units expressed as μmol per gram of cell wall residue (CWR). In panels a-d, all values shown for the HCT-RNAi lines are significantly different (P < 0.05) from the wild-type control (WT) plants. For panels e–h, statistically significant differences (P < 0.05) are shown with an asteriskBack to article page