Targeting hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase for lignin modification in Brachypodium distachyon

Table 1 Kinetics of recombinant HCTs in the forward and reverse directions

Enzyme	Km (µM⁻¹)	Kcat (s⁻¹)^a	Kcat/Km (s⁻¹ µM⁻¹)
HCT forward: 4-coumaroyl CoA (varying substrate) + shikimic acid (saturating substrate)
AtHCT1	18.5	17.5	0.95
BdHCT1	32.2	25.3	0.79
BdHCT2	55.2	20.5	0.37
MtHCT1	40.6	40.8	1.01
MtHCT2	44.3	48.2	1.01
*PvHCT1	19.1	60.6	3.13
*PvHCT2	5.1	10.3	2.00
HCT reverse: caffeoyl shikimate (varying substrate) + CoA (saturating substrate)
AtHCT1	225.0	17.0	0.076
BdHCT1	511.8	41.9	0.082
BdHCT2	63.4	12.0	0.189
MtHCT1	147.7	25.9	0.175
MtHCT2	139.3	31.1	0.223
*PvHCT1	60.6	7.5	0.123
*PvHCT2	56.7	5.2	0.090

*Data taken from [27] Escamilla-Treviño et al., 2014
^aKcat values are apparent values in view of the need to estimate purity of enzyme preparations by gel scanning and image analysis

ISSN: 2731-3654