|
Enzyme
|
Km (µM−1)
|
Kcat (s−1)a
|
Kcat/Km (s−1 µM−1)
|
|---|
|
HCT forward: 4-coumaroyl CoA (varying substrate) + shikimic acid (saturating substrate)
|
|
AtHCT1
|
18.5
|
17.5
|
0.95
|
|
BdHCT1
|
32.2
|
25.3
|
0.79
|
|
BdHCT2
|
55.2
|
20.5
|
0.37
|
|
MtHCT1
|
40.6
|
40.8
|
1.01
|
|
MtHCT2
|
44.3
|
48.2
|
1.01
|
|
*PvHCT1
|
19.1
|
60.6
|
3.13
|
|
*PvHCT2
|
5.1
|
10.3
|
2.00
|
|
HCT reverse: caffeoyl shikimate (varying substrate) + CoA (saturating substrate)
|
|
AtHCT1
|
225.0
|
17.0
|
0.076
|
|
BdHCT1
|
511.8
|
41.9
|
0.082
|
|
BdHCT2
|
63.4
|
12.0
|
0.189
|
|
MtHCT1
|
147.7
|
25.9
|
0.175
|
|
MtHCT2
|
139.3
|
31.1
|
0.223
|
|
*PvHCT1
|
60.6
|
7.5
|
0.123
|
|
*PvHCT2
|
56.7
|
5.2
|
0.090
|
- *Data taken from [27] Escamilla-Treviño et al., 2014
- aKcat values are apparent values in view of the need to estimate purity of enzyme preparations by gel scanning and image analysis
