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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum

Fig. 1

Screening transgenic T×430 sorghum lines for the presence of isomaltulose (IM) in stem juice. a High-performance liquid chromatography (HPLC) profiles. Black curve: Standard solutions contained glucose, fructose, sucrose, trehalulose and IM; Pink curve: Diluted (16,000x) juice from transgenic sorghum stalk internode 4 showing isomaltulose (last peak #5) was accumulated beyond glucose, fructose and sucrose; Blue curve: Diluted (16,000x) juice from a parent control sorghum stalk showing no isomaltulose accumulated. b isomaltulose concentrations in juice from the internode 4 of the transgenic lines. The line’s label starts with A driven by A1 promoter and with L driven by LSG2 promoter. The plants were the first vegetative generation from tissue culture with around 7 internodes when sampled 20-day post-anthesis. A horizontal line was drawn on the highest total sugar content (sucrose equivalent) among the five T×430 plants (Controls)

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