Fig. 2

Effect of ACY1 on Mn²⁺/DMF-stimulated cellulase overexpression. a and b. pNPCase activity/mg biomass (a) and CMCase activity/mg biomass (b) of T. reesei QM6a and Δacy1 strains supplemented with 10 mM Mn²⁺ or 1% DMF. c and d. The relative expression levels of cbh1 (c) and egl1 (d) in T. reesei QM6a and Δacy1 strains cultured in medium supplemented with 10 mM Mn²⁺ or 1% DMF. No addition (QM6a-no addition and Δacy1-no addition) were used as controls. The QM6a culture with no addition at 24 h was used as the reference sample. Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences from the control (*p < 0.05, Student’s t test)