Fig. 4

Effect of PLC-E on Mn²⁺-stimulated cellulase overexpression. a and b. pNPCase activity/mg biomass (a) and CMCase activity/mg biomass (b) of T. reesei QM6a and Δplc-e strains supplemented with 10 mM Mn²⁺. c and d The relative expression levels of cbh1 (c) and egl1 (d) in T. reesei QM6a and Δplc-e strains cultured in medium supplemented with 10 mM Mn²⁺. No addition (QM6a-no addition and Δplc-e-no addition) was used as the respective control. The Δplc-e-no addition at 24 h was used as the reference sample. Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences from the control (*p < 0.05, Student’s t test)