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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries

Fig. 4

Examples of the application of anti-glycan mAbs for the characterization of cell walls. Labeling of cross-sections of a Brachypodium internode: ac BS-400-3 antibody specific for mixed-linkage glucan (MLG; green signal). df LM11 specific for arabinoxylan (secondary anti-rat antibody conjugated to AlexaFluor488; green signal). b, e The sections were counterstained with Calcofluor White (CW; blue signal). Note the different distribution of the two epitopes. c,f Overlay of antibody and CW channels. Scale bars = 50 μm. Authors’ own unpublished work

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