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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries

Fig. 6

Scheme of the principle of click-labeling polysaccharides, common click reactions in plants, confocal image of a click-labeling experiment. a Scheme of the principle of click-labeling polysaccharides. The inset depicts an example of a functionalized and acetylated aldopyranose, the position of functionalization is not specified, and could be on of any of the hydroxy groups. \(\text {R}^M\) monosaccharide, \(\text {R}^F\) fluorophore, R either monosaccharide or fluorophore. be Common click reactions used in plant studies: b Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC), c strain-promoted alkyne-azide cycloaddition (SPAAC), d Inverse electron demand Diels-Alder (\(\text {DA}_{inv}\)). e Example of an incorporation and click reaction based on [164]. Note the small size of the triazole connecting the monosaccharide and the fluorophore. f Confocal (top) and corresponding bright-field image (BF, bottom) of fucose alkyne CuAAC click-labeled Arabidopsis root hairs (RH) and root, scale bar = 20 μm, authors’ own unpublished work, based on [164]

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