Skip to main content

Table 2 Saccharide composition of onion epidermal wall pre-extracted with chloroform and met/TFA/Saeman’s hydrolysis

From: Saccharide analysis of onion outer epidermal walls

  µg/mg dry weight %
Trial 1 Trial 2 Trial 3 mean Total
Chl + mTFA Sae Chl + mTFA Sae Chl + mTFA Sae Chl + mTFA Sae
Glc 26.15 360.28 7.57 151.44 20.2 172.6 18.0 (4.5) 228.1 (54.2) 246.1 34
GalA 422.66 16.00 144.28 65.46 320.4 22.1 295.8 (66.4) 34.5 (12.7) 330.3 45
Gal 101.77 5.01 4.42 4.84 114.0 4.3 73.4 (28.3) 4.7 (0.2) 78.1 11
Xyl/Man* 44.27 17.05 4.57 11.80 38.0 8.7 29.0 (10.1) 12.5 (2.0) 41.5 6
Rha 15.49 0.67 1.98 0.76 14.4 0.7 10.6 (3.5) 0.7 (0.02) 11.3 2
Ara 15.78 0.67 0.83 0.72 11.3 0.7 9.3 (3.6) 0.7 (0.02) 10.0 1
Fuc 4.97 6.72 3.81 0.00 4.4 0.0 4.4 (0.3) 2.2 (1.8) 6.6 1
GlcA 1.9 0.0 0.0 0.0 5.0 0.0 2.3 (1.3) 0.0 (0.0) 2.3 0.3
Total 633.0 406.4 167.5 235.0 527.8 209.1 442.8 283.5 726.3 100
  1. Chl + mTFA, peel chloroform pretreated followed by methanolysis with TFA; Sae, 2-step sulfuric acid (Saeman's hydrolysis) on chloroform pretreated residue; Xyl/Man*, HPAEC-PAD parameters did not separate xylose and mannose peaks. µg/mg values were adjusted using correction factors listed in “Materialsandmethods”; (SEM), standard error of mean of 3 biological replicates