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Table 4 Saccharide composition of onion epidermal wall pretreated with chloroform and Driselase enzyme followed by met/TFA/Saeman’s hydrolysis

From: Saccharide analysis of onion outer epidermal walls

  µg/mg dry weight %
Trial 1 Trial 2 Trial 3 mean  
Chl + Dri + mTFA Sae Chl + Dri + mTFA Sae Chl + Dri + mTFA Sae Chl + Dri + mTFA Sae Total
Glc 41.0 63.4 20.9 9.0 97.6 6.9 53.2 (18.7) 26.5 (15.1) 79.6 19
GalA 190.3 22.7 208.3 4.9 287.4 26.9 228.7 (24.3) 18.2 (5.5) 246.8 60
Gal 32.3 4.2 14.7 0.1 84.6 2.2 43.9 (17.2) 2.1 (1.0) 46.0 11
Xyl/Man* 13.3 9.7 6.3 2.0 28.5 2.7 16.0 (5.4) 4.8 (2.0) 20.8 5
Rha 6.0 0.3 5.5 0.0 13.2 0.5 8.3 (2.0) 0.3 (0.1) 8.5 2
Ara 5.1 0.8 1.8 1.2 9.5 0.4 5.5 (1.8) 0.8 (0.2) 6.3 2
Fuc 1.4 1.7 0.0 0.1 3.8 0.0 1.7 (0.9) 0.6 (0.5) 2.3 0.6
GlcA 0.0 0.0 0.0 0.0 2.8 0.0 0.9 (0.8) 0.0 (0.0) 0.9 0.2
Total 289.4 102.8 257.5 17.2 527.5 39.6 358.1 53.2 411.3 100
  1. Chl + Dri + mTFA, peel is pretreated with chloroform and then Driselase digested. Soluble sugars are hydrolyzed by methanolysis with TFA; Sae, 2-step sulfuric acid (Saeman’s hydrolysis) on the residue; Xyl/Man*, HPAEC-PAD parameters did not separate xylose and mannose peaks. µg/mg values were adjusted using correction factors listed in “Materialsandmethods”. (SEM), standard error of mean of 3 biological replicates