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Table 4 Saccharide composition of onion epidermal wall pretreated with chloroform and Driselase enzyme followed by met/TFA/Saeman’s hydrolysis

From: Saccharide analysis of onion outer epidermal walls

 

µg/mg dry weight

%

Trial 1

Trial 2

Trial 3

mean

 

Chl + Dri + mTFA

Sae

Chl + Dri + mTFA

Sae

Chl + Dri + mTFA

Sae

Chl + Dri + mTFA

Sae

Total

Glc

41.0

63.4

20.9

9.0

97.6

6.9

53.2 (18.7)

26.5 (15.1)

79.6

19

GalA

190.3

22.7

208.3

4.9

287.4

26.9

228.7 (24.3)

18.2 (5.5)

246.8

60

Gal

32.3

4.2

14.7

0.1

84.6

2.2

43.9 (17.2)

2.1 (1.0)

46.0

11

Xyl/Man*

13.3

9.7

6.3

2.0

28.5

2.7

16.0 (5.4)

4.8 (2.0)

20.8

5

Rha

6.0

0.3

5.5

0.0

13.2

0.5

8.3 (2.0)

0.3 (0.1)

8.5

2

Ara

5.1

0.8

1.8

1.2

9.5

0.4

5.5 (1.8)

0.8 (0.2)

6.3

2

Fuc

1.4

1.7

0.0

0.1

3.8

0.0

1.7 (0.9)

0.6 (0.5)

2.3

0.6

GlcA

0.0

0.0

0.0

0.0

2.8

0.0

0.9 (0.8)

0.0 (0.0)

0.9

0.2

Total

289.4

102.8

257.5

17.2

527.5

39.6

358.1

53.2

411.3

100

  1. Chl + Dri + mTFA, peel is pretreated with chloroform and then Driselase digested. Soluble sugars are hydrolyzed by methanolysis with TFA; Sae, 2-step sulfuric acid (Saeman’s hydrolysis) on the residue; Xyl/Man*, HPAEC-PAD parameters did not separate xylose and mannose peaks. µg/mg values were adjusted using correction factors listed in “Materialsandmethods”. (SEM), standard error of mean of 3 biological replicates