|
µg/mg dry weight
|
%
|
---|
Trial 1
|
Trial 2
|
Trial 3
|
mean
| |
---|
Chl + Dri + mTFA
|
Sae
|
Chl + Dri + mTFA
|
Sae
|
Chl + Dri + mTFA
|
Sae
|
Chl + Dri + mTFA
|
Sae
|
Total
|
---|
Glc
|
41.0
|
63.4
|
20.9
|
9.0
|
97.6
|
6.9
|
53.2 (18.7)
|
26.5 (15.1)
|
79.6
|
19
|
GalA
|
190.3
|
22.7
|
208.3
|
4.9
|
287.4
|
26.9
|
228.7 (24.3)
|
18.2 (5.5)
|
246.8
|
60
|
Gal
|
32.3
|
4.2
|
14.7
|
0.1
|
84.6
|
2.2
|
43.9 (17.2)
|
2.1 (1.0)
|
46.0
|
11
|
Xyl/Man*
|
13.3
|
9.7
|
6.3
|
2.0
|
28.5
|
2.7
|
16.0 (5.4)
|
4.8 (2.0)
|
20.8
|
5
|
Rha
|
6.0
|
0.3
|
5.5
|
0.0
|
13.2
|
0.5
|
8.3 (2.0)
|
0.3 (0.1)
|
8.5
|
2
|
Ara
|
5.1
|
0.8
|
1.8
|
1.2
|
9.5
|
0.4
|
5.5 (1.8)
|
0.8 (0.2)
|
6.3
|
2
|
Fuc
|
1.4
|
1.7
|
0.0
|
0.1
|
3.8
|
0.0
|
1.7 (0.9)
|
0.6 (0.5)
|
2.3
|
0.6
|
GlcA
|
0.0
|
0.0
|
0.0
|
0.0
|
2.8
|
0.0
|
0.9 (0.8)
|
0.0 (0.0)
|
0.9
|
0.2
|
Total
|
289.4
|
102.8
|
257.5
|
17.2
|
527.5
|
39.6
|
358.1
|
53.2
|
411.3
|
100
|
- Chl + Dri + mTFA, peel is pretreated with chloroform and then Driselase digested. Soluble sugars are hydrolyzed by methanolysis with TFA; Sae, 2-step sulfuric acid (Saeman’s hydrolysis) on the residue; Xyl/Man*, HPAEC-PAD parameters did not separate xylose and mannose peaks. µg/mg values were adjusted using correction factors listed in “Materialsandmethods”. (SEM), standard error of mean of 3 biological replicates