Column | HPAEC-PAD | Â | GC/MS | Â |
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A | B | C | D | Â | E | F | G |
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mTFA/S | Chl + mTFA/S | Dri + mTFA/S | Chl/Dris + mTFA/S | Average | TMS | Methyl alditols | Col E&F MERGED |
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Glc | 25 | 35 | 17 | 20 | 24 | 2 | 44 | 23 |
GalA/GlcA | 49 | 43 | 58 | 58 | 52 | 75 | 7 | 51 |
Gal | 14 | 11 | 14 | 12 | 12 | 17 | 27 | 14 |
Xyl | 5 | 5 | 4 | 4 | 5 | 3 | 9 | 5 |
Rha | 3 | 2 | 3 | 2 | 2 | 1 | 3 | 2 |
Ara | 2 | 2 | 3 | 2 | 2 | 1 | 4 | 2 |
Fuc | 1 | 1 | 1 | 1 | 1 | – | – | – |
Man | 2 | 2 | 2 | 2 | 2 | 2 | 6 | 3 |
Total | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
- mol%, determined using 'total µg/mg' values for each experiment. Column G merges the GalA/GlcA µg/mg amount with the methyl alditol amounts. mTFA/S, methanolysis followed by TFA and Saeman’s hydrolysis; Chl + mTFA/S, chloroform pretreated peel, methanolysis followed by TFA and Saeman’s hydrolysis; Dri + mTFA/S, Driselase digested, methanolysis followed by TFA and Saeman's hydrolysis; Chl/Dri + mTFA/S, chloroform pretreat, Driselase digested, methanolysis followed by TFA and Saeman’s hydrolysis