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Table 6 Comparative summary of monosaccharides in the onion epidermal wall (mol%)

From: Saccharide analysis of onion outer epidermal walls

Column

HPAEC-PAD

 

GC/MS

 

A

B

C

D

 

E

F

G

mTFA/S

Chl + mTFA/S

Dri + mTFA/S

Chl/Dris + mTFA/S

Average

TMS

Methyl alditols

Col E&F MERGED

Glc

25

35

17

20

24

2

44

23

GalA/GlcA

49

43

58

58

52

75

7

51

Gal

14

11

14

12

12

17

27

14

Xyl

5

5

4

4

5

3

9

5

Rha

3

2

3

2

2

1

3

2

Ara

2

2

3

2

2

1

4

2

Fuc

1

1

1

1

1

–

–

–

Man

2

2

2

2

2

2

6

3

Total

100

100

100

100

100

100

100

100

  1. mol%, determined using 'total µg/mg' values for each experiment. Column G merges the GalA/GlcA µg/mg amount with the methyl alditol amounts. mTFA/S, methanolysis followed by TFA and Saeman’s hydrolysis; Chl + mTFA/S, chloroform pretreated peel, methanolysis followed by TFA and Saeman’s hydrolysis; Dri + mTFA/S, Driselase digested, methanolysis followed by TFA and Saeman's hydrolysis; Chl/Dri + mTFA/S, chloroform pretreat, Driselase digested, methanolysis followed by TFA and Saeman’s hydrolysis