Fig. 4
From: Understanding and exploiting the fatty acid desaturation system in Rhodotorula toruloides
Characterization of FAD2. a GCMS chromatographs of FAMEs from WT and FAD2 deletion mutant (fad2Δ). + 18:2 indicates cells cultured in medium supplemented with 18:2. b Complementation of fatty acid production defect of fad2Δ by overexpression of known heterologous Δ12- and/or Δ15-fatty acid desaturase. WT wild-type strain, fad2MF2 fad2Δ expressing the codon-optimized gene encoding M. alpina Δ12 FAD (MaFAD2-2), fad2LF3 fad2Δ expressing the codon-optimized flax Δ15 FAD (LuFAD3-2), fad2ML fad2Δ expressing both MaFAD2-2 and LuFAD3-2. c Fatty acid profiles of WT, fad2Δ, fad2FAD2 and fad2FAD4. %TFA represents weight percentage of total fatty acids. Error bars represent standard derivations of triplicates