Fig. 7

Identification and analyses of OLE2. a qRT-PCR analysis of OLE1 and OLE2 expression. Seed cultures were water-washed twice and inoculated in YNB broth (without carbon source) supplemented with different fatty acids (10 g/L) and Tergitol NP40 (1%, w/v), and cultured at 28 °C, 280 rpm for 8 h. Glucose (10 g/L) was used as the control. The values presented are the relative expression against ACT1 mRNA (2-ΔCt method). Error bars represent the standard derivations of triplicates. b Sequence alignment of Ole1 (partial) and Ole2. c Fatty acid profiles of WT and ole2Δ. 16:1 was shown in the inlet plot