Table 2 Strains and plasmids used in this study
From: Understanding and exploiting the fatty acid desaturation system in Rhodotorula toruloides
Strain/plasmid | Characteristics | Source |
|---|---|---|
Strains | ||
 R. toruloides ATCC 10657 | R. toruloides host | ATCC |
 C3 | A haploid strain isolated from Singapore | This study |
 ole1Δ | OLE1::HygR in C3 background | This study |
 R. toruloides Rt1ck | Δku70e, ku70Δ::loxPa, marker free, designed as wild-type strain | [47] |
 OLE1GPD1 | ku70Δ::loxP car2Δ::PGPD1-OLE1-HygRb | This report |
 fad2Δ | ku70Δ::loxP fad2Δ::HygR | This report |
 fad2e | ku70Δ::loxP fad2Δ::loxP | This report |
 fad2C | ku70Δ::loxP fad4Δ::loxP car2Δ::PGPD1-FAD2-HygR (alias fad2FAD2) | This report |
 fad4Δ | ku70Δ::loxP fad4Δ::HygR | This report |
 fad4e | ku70Δ::loxP fad4Δ::loxP | This report |
 fad4FAD4 | ku70Δ::loxP fad4Δ::loxP car2Δ::PGPD1-FAD4-HygR (alias fad4FAD4) | This report |
 fad4FAD4a | ku70Δ::loxP fad4Δ::loxP car2Δ::FAD4 allele-HygR | This report |
 fad24Δ | ku70Δ::loxP fad4::loxP fad2Δ::HygR | This report |
 fad24e | ku70Δ::loxP fad4Δ::loxP fad2Δ::loxP | This report |
 fad2FAD4 | ku70Δ::loxP fad2Δ::loxP car2Δ::PGPD1-FAD4-HygR | This report |
 fad4FAD2 | ku70Δ::loxP fad4Δ::loxP car2Δ::PGPD1-FAD2-HygR | This report |
 fad24FAD2 | ku70Δ::loxP fad4Δ::loxP fad2Δ::loxP car2Δ::PGPD1-FAD2-HygR | This report |
 fad24FAD4 | ku70Δ::loxP fad4Δ::loxP fad2Δ::loxP car2Δ::PGPD1-FAD4-HygR | This report |
 fad2MF2 | ku70Δ::loxP fad2Δ::loxP car2Δ::PGPD1-MaFAD2-2-HygR | This report |
 fad2LF3 | ku70Δ::loxP fad2Δ::loxP car2Δ::PGPD1-LuFAD3-2-HygR | This report |
 fad2ML | ku70Δ::loxP fad2Δ::loxP car2Δ::PGPD1-MaFAD2-2-PGPD1-LuFAD3-2-HygR | This report |
 fad2OLE1 | ku70Δ::loxP fad2Δ::loxP car2Δ::PLPD1in-OLE1-HygR | This report |
 fad24OLE1 | ku70Δ::loxP fad4Δ::loxP fad2Δ::loxP car2Δ::PLPD1in-OLE1-HygR | This report |
 fad2MF26 | ku70Δ::loxP fad2Δ::loxP car2Δ::PLDP1in-MaFAD2-2-PLDP1in-MaFAD6-2-HygR | This report |
 fad24MF26 | ku70Δ::loxP fad2Δ::loxP fad4Δ::loxP car2Δ::PLDP1in-MaFAD2-2-PLDP1in-MaFAD6-2-HygR | This report |
 A. tumefaciens AGL1 | Agrobacterium host for ATMT | [71] |
 E. coli XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac, E. coli host for routine DNA manipulation | Stratagene, USA |
Plasmids | ||
 pEX2 | SpRc, binary vector pZP200 derivative | [69] |
 pKC2 | SpR, pEX2 derivative, CAR2L-PGPD1-RtGFP-HygR-CAR2Rd, for promoter analysis, gene expression and CAR2 locus integration | [68] |
 pKCL2 | SpR, pKCL2 derivative, CAR2L-PGPD1-RtLUC2-HygR-CAR2R, for promoter analysis, gene expression and CAR2 locus integration | [68] |
 pKCL25 | SpR, pKCL2 derivative, CAR2L-PGPD1-176-RtLUC2-HygR-CAR2R, for promoter analysis and CAR2 locus integration | This report |
 pKCL254 | SpR, pKCL2 derivative, CAR2L-ORE1-PGPD1-176-RtLUC2-35T-HygR-CAR2R, for promoter reporter assay and CAR2 locus integration | This report |
 pKCL255 | SpR, pKCL2 derivative, CAR2L-ORE2-PGPD1-176-RtLUC2-35T-HygR-CAR2R, for promoter reporter assay and CAR2 locus integration | This report |
 pKCL256 | SpR, pKCL2 derivative, CAR2L-ORE1m-PGPD1-176-RtLUC2-35T-HygR-CAR2R, for promoter reporter assay and CAR2 locus integration | This report |
 pKCLF66 | SpR, pKCL2 derivative, CAR2L-POLE1-641-RtLUC2-35T-HygR-CAR2R, for promoter reporter assay and CAR2 locus integration | This report |
 pKCLF661 | SpR, pKCL2 derivative, CAR2L-POLE1-641-RtLUC2-TOLE1-HygR-CAR2R, for promoter reporter assay and CAR2 locus integration | This report |
 pKCLP4 | SpR, pKCL2 derivative, CAR2L-PLDP1in-RtLUC2-HygR-CAR2R, for LDP1in promoter driving gene overexpression and CAR2 locus integration | [26] |
 pKOFAD2 | SpR, pEX2 derivative, FAD2L-HygR-FAD2R, for deletion of FAD2 | This report |
 pKOFAD4 | SpR, pEX2 derivative, FAD4L-HygR-FAD4R, for deletion of FAD4 | This report |
 pNE1OLE1ca | SpR, CAR2L-OLE1 allele-HygR-CAR2R, for complementation of ole1Δ | This report |
 pKC2FAD4 | SpR, CAR2L-PGPD1-FAD4-HygR-CAR2R, for overexpression of FAD4 | This report |
 pKC2FAD4a | SpR, pKC2 derivative, HygR-FAD4 allele, for complementation | This report |
 pKC2MF2 | SpR, pKC2 derivative, CAR2L-PGPD1-MaFAD2-2-HygR-CAR2R, for overexpression of MaFAD2-2 | This report |
 pKC2LF3 | SpR, pKC2 derivative, CAR2L-PGPD1-LuFAD3-2-HygR-CAR2R, for overexpression of LuFAD3-2 | This report |
 pKC2ML | SpR, pKC2 derivative, CAR2L-PGPD1-MaFAD2-2-PGPD1-LuFAD3-2-HygR-CAR2R, for overexpression of MaFAD2-2 and LuFAD3-2 | This report |
 pKP4OLE1 | SpR, pKCLP4 derivative, PLDP1in-RtLUC2-HygR, for overexpression of OLE1 | This report |
 pKP4MF2 | SpR, pKCLP4derivative, CAR2L-PLDP1in-MaFAD2-2-HygR-CAR2R, for overexpression of MaFAD2-2 | This report |
 pKP4MF6 | SpR, pKCLP4 derivative, CAR2L-PLDP1in-MaFAD6-2-HygR-CAR2R, for overexpression of MaFAD6-2 | This report |
 pKP4MF26 | SpR, pKCLP4 derivative, PLDP1in-MaFAD2-2-PLDP1in- MaFAD6-2-HygR, for overexpression of MfFAD2-2 and MfFAD6-2 | This report |