Fig. 4
ApV1 expression from PHpFMD exceeds expression from PAOX1 and PDI co-expression with BDP PHpFMD-HpMOX shows the highest ApV1 yields. ApV1 expression from MDPs PAOX1 and PHpFMD with AppA (PAOX1) with phytase activity (mU/mL) shown on the secondary axis (a). ApV1 co-expression with HAC1 from the BDPs PAOX1-CAT1, PDAS1/2 and PHpFMD-HpMOX (b). ApV1 co-expression with PDI, MPDI and EUG1 under the BDP PHpFMD-HpMOX (c). ApV1 co-expression with or without PDI and flavoproteins ERO1 and ERV2 (d). Phytase expression was determined by the p-NPP assay. AppA (PHpFMD) is included for comparison. All strains were induced with methanol at 65 h and consecutive methanol additions until 132 h. Data are represented as mean values ± standard deviation (n = 3)