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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Fig. 5

Removal of native disulfide bonds from ApV1 (variants ApV2, ApV3, ApV4 and ApV5) does not improve phytase production. Diagram showing mutated cysteine residues in each ApV1 variants (a). ApV2, ApV3, ApV4 and ApV5 phytase activity (mU/mL) compared to ApV1 (b). Phytase activity was determined by the p-NPP assay (Abs 410 nm). Data are represented as mean values ± standard deviation (n = 3)

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