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Table 1 Kinetic parameters for ApV1 thermostable phytase compared to AppA phytase at 37 °C

From: Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

 

Specific activity (103 U/g of protein)*

KM (mM)*

kcat (min−1)*

kcat/KM (min−1 M−1)*

Phytic acid assay

 AppA phytase

1150 ± 138

0.83 ± 0.04

3.91 × 105 ± 0.51 × 105

3.47 × 108 ± 0.34 × 108

 ApV1 phytase

1273 ± 238

0.82 ± 0.04

2.65 × 105 ± 0.37 × 105

3.23 × 108 ± 0.28 × 108

p-NPP assay

 AppA phytase

1075 ± 40

10.63 ± 1.94

2.02 × 105 ± 0.64 × 105

1.57 × 107 ± 0.22 × 107

 ApV1 phytase

987 ± 42

11.34 ± 0.48

2.45 × 105 ± 0.13 × 105

1.72 × 107 ± 0.86 × 107

  1. *No significant statistical difference between phytases for phytic acid or p-NPP assays (p ≤ 0.05). Values correspond to biological triplicates of his-tag purified AppA and ApV1 phytases