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Table 3 Fold change in ApV1 phytase activity at 132 h relative to ApV1 (PAOX1), ApV1 (PHpFMD) or ApV1-PDI (PHpFMD-HpMOX)

From: Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Strain

Fold change relative to ApV1 (PAOX1)

Fold change relative to ApV1 (PHpFMD)

Fold change relative to ApV1-PDI (PHpFMD-HpMOX)

AppA (PHpFMD)

14.01 ± 0.06*

11.91 ± 0.05**

0.96 ± 0.01

ApV1 (PAOX1)

–

0.85 ± 0.04**

0.07 ± 0.01***

ApV1 (PHpFMD)

1.18 ± 0.03*

–

0.08 ± 0.01***

ApV1-HAC1 (PHpFMD-MOX)

2.90 ± 0.05*

2.46 ± 0.05**

0.20 ± 0.01***

ApV1-HAC1 (PAOX1- CAT1)

1.57 ± 0.15*

1.34 ± 0.13

0.11 ± 0.01***

ApV1-HAC1 (PDAS1/2)

1.58 ± 0.05*

1.35 ± 0.01

0.11 ± 0.01***

ApV1-PDI (PHpFMD-MOX)

14.63 ± 1.00*

12.44 ± 0.90**

–

APV1-MPDI (PHpFMD-MOX)

1.66 ± 0.50*

1.41 ± 0.10**

0.11 ± 0.03***

APV1-EUG1 (PHpFMD-MOX)

0.57 ± 0.11*

0.48 ± 0.10**

0.04 ± 0.01***

APV1-ERO1 (PHpFMD-MOX)

0.74 ± 0.14*

0.87 ± 0.17

0.06 ± 0.03***

APV1-ERV2 (PHpFMD-MOX)

1.27 ± 0.01*

1.08 ± 0.01

0.09 ± 0.01***

APV1-PDI-ERO1 (PGAP)

15.95 ± 0.05*

13.56 ± 0.05**

1.09 ± 0.03

APV1-PDI-ERV2 (PGAP)

15.37 ± 0.03*

13.07 ± 0.02**

1.05 ± 0.05

ApV1-PDI ots1 (PHpFMD-MOX)

5.82 ± 0.82*

4.95 ± 0.07**

0.40 ± 0.06***

ApV1-PDI suc2 (PHpFMD-MOX)

2.06 ± 0.01*

2.21 ± 0.05**

0.18 ± 0.01***

ApV1-PDI pho1 (PHpFMD-MOX)

1.73 ± 0.07*

1.47 ± 0.06**

0.12 ± 0.01***

  1. aAll strains had a single copy of the integrated cassette (CNV = 1) as determined by ddPCR
  2. *Significantly different from ApV1 (PAOX1) (p value ˂ 0.05)
  3. **Significantly different from ApV1 (PHpFMD) (p value ˂ 0.05)
  4. ***Significantly different from ApV1-PDI (PHpFMD-HpMOX) (p value ˂ 0.05)