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Table 3 Fold change in ApV1 phytase activity at 132 h relative to ApV1 (PAOX1), ApV1 (PHpFMD) or ApV1-PDI (PHpFMD-HpMOX)

From: Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Strain Fold change relative to ApV1 (PAOX1) Fold change relative to ApV1 (PHpFMD) Fold change relative to ApV1-PDI (PHpFMD-HpMOX)
AppA (PHpFMD) 14.01 ± 0.06* 11.91 ± 0.05** 0.96 ± 0.01
ApV1 (PAOX1) 0.85 ± 0.04** 0.07 ± 0.01***
ApV1 (PHpFMD) 1.18 ± 0.03* 0.08 ± 0.01***
ApV1-HAC1 (PHpFMD-MOX) 2.90 ± 0.05* 2.46 ± 0.05** 0.20 ± 0.01***
ApV1-HAC1 (PAOX1- CAT1) 1.57 ± 0.15* 1.34 ± 0.13 0.11 ± 0.01***
ApV1-HAC1 (PDAS1/2) 1.58 ± 0.05* 1.35 ± 0.01 0.11 ± 0.01***
ApV1-PDI (PHpFMD-MOX) 14.63 ± 1.00* 12.44 ± 0.90**
APV1-MPDI (PHpFMD-MOX) 1.66 ± 0.50* 1.41 ± 0.10** 0.11 ± 0.03***
APV1-EUG1 (PHpFMD-MOX) 0.57 ± 0.11* 0.48 ± 0.10** 0.04 ± 0.01***
APV1-ERO1 (PHpFMD-MOX) 0.74 ± 0.14* 0.87 ± 0.17 0.06 ± 0.03***
APV1-ERV2 (PHpFMD-MOX) 1.27 ± 0.01* 1.08 ± 0.01 0.09 ± 0.01***
APV1-PDI-ERO1 (PGAP) 15.95 ± 0.05* 13.56 ± 0.05** 1.09 ± 0.03
APV1-PDI-ERV2 (PGAP) 15.37 ± 0.03* 13.07 ± 0.02** 1.05 ± 0.05
ApV1-PDI ots1 (PHpFMD-MOX) 5.82 ± 0.82* 4.95 ± 0.07** 0.40 ± 0.06***
ApV1-PDI suc2 (PHpFMD-MOX) 2.06 ± 0.01* 2.21 ± 0.05** 0.18 ± 0.01***
ApV1-PDI pho1 (PHpFMD-MOX) 1.73 ± 0.07* 1.47 ± 0.06** 0.12 ± 0.01***
  1. aAll strains had a single copy of the integrated cassette (CNV = 1) as determined by ddPCR
  2. *Significantly different from ApV1 (PAOX1) (p value ˂ 0.05)
  3. **Significantly different from ApV1 (PHpFMD) (p value ˂ 0.05)
  4. ***Significantly different from ApV1-PDI (PHpFMD-HpMOX) (p value ˂ 0.05)