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Table 2 Substrate oxidation spectrum of PcLac1 and PcLac2

From: Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis

  Substrate PcLac1 PcLac2
Hydroxybenzenes
1 Phenol
2 Catechol  +   +  + 
3 Resorcinol
4 Hydroquinone  +   + 
5 Chlorocatechol  + 
6 Pyrogallol  +   + 
Methoxyphenols
7 Guaiacol  ±   ± 
8 2.6-Dimethoxyphenol  +   + 
Aromatic alcohols
9 3.4-Dimethoxybenzyl alcohol  + 
Phenethyl alcohols
10 Tyrosol
Aromatic amines
11 Aniline
12 Tyrosine
Phenolic aldehydes
13 Vanillin
Flavonoids
14 Quercetin  +  +   +  + 
Hydroxycinnamic acids
15 Caffeic acid  +   + 
16 Ferulic acid  +   + 
17 Sinapic acid  +   + 
18 p-Coumaric acid
Hydroxybenzoic acids
19 Vanillic acid  + 
20 Gallic acid  + 
Aromatic azo compounds
21 ABTS  +  +  +   +  +  + 
Other acids
22 Ascorbic acid
Cresols
23 o-Cresol
24 m-Cresol
25 p-Cresol  + 
  1. Activity, in terms of absorbance difference in the recorded UV/Vis spectrum between reaction and blank, is indicated with ( +) when positive (indicating absorbance difference in the spectrum maxima), with (−) when negative and with ( ±) when ambiguous. Multiple ( +) signs indicate differences higher than 1 (+ +) or 2 (+ + +) absorbance units
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