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Table 2 Substrate oxidation spectrum of PcLac1 and PcLac2

From: Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis

 

Substrate

PcLac1

PcLac2

Hydroxybenzenes

1

Phenol

−

−

2

Catechol

 + 

 +  + 

3

Resorcinol

−

−

4

Hydroquinone

 + 

 + 

5

Chlorocatechol

−

 + 

6

Pyrogallol

 + 

 + 

Methoxyphenols

7

Guaiacol

 ± 

 ± 

8

2.6-Dimethoxyphenol

 + 

 + 

Aromatic alcohols

9

3.4-Dimethoxybenzyl alcohol

−

 + 

Phenethyl alcohols

10

Tyrosol

−

−

Aromatic amines

11

Aniline

−

−

12

Tyrosine

−

−

Phenolic aldehydes

13

Vanillin

−

−

Flavonoids

14

Quercetin

 +  + 

 +  + 

Hydroxycinnamic acids

15

Caffeic acid

 + 

 + 

16

Ferulic acid

 + 

 + 

17

Sinapic acid

 + 

 + 

18

p-Coumaric acid

−

−

Hydroxybenzoic acids

19

Vanillic acid

−

 + 

20

Gallic acid

−

 + 

Aromatic azo compounds

21

ABTS

 +  +  + 

 +  +  + 

Other acids

22

Ascorbic acid

−

−

Cresols

23

o-Cresol

−

−

24

m-Cresol

−

−

25

p-Cresol

−

 + 

  1. Activity, in terms of absorbance difference in the recorded UV/Vis spectrum between reaction and blank, is indicated with ( +) when positive (indicating absorbance difference in the spectrum maxima), with (−) when negative and with ( ±) when ambiguous. Multiple ( +) signs indicate differences higher than 1 (+ +) or 2 (+ + +) absorbance units