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Table 4 Michaelis–Menten kinetic parameters calculated for PcLac1 and PcLac2

From: Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis

Enzyme

Substrate

Vmax (Units/mg)

kcat (min−1)

KM (μΜ)

Kcat/KM (μM−1 min−1)

PcLac1

ABTS

2.38 ± 0.06

95.2 ± 2.3

60 ± 7

1.57 ± 0.17

2.6-DMP

0.36 ± 0.01

14.2 ± 0.5

672 ± 56

0.021 ± 0.002

PcLac2

ABTS

4.6 ± 0.1

368.7 ± 8.8

165 ± 15

2.24 ± 0.21

2.6-DMP

0.041 ± 0.002

3.3 ± 0.2

1070 ± 105

0.0030 ± 0.0003

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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