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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation

Fig. 1

The scheme for XYR1 overexpression and ACE1 disruption. a A scheme for C30OExyr1 construction. b A scheme for C30OExyr1Δace1 construction. c PCR analysis of recombinant C30OExyr1 and C30OExyr1Δace1. Lanes 1, 3, and 5 represent the PCR results of C30OExyr1, and Lanes 2, 4, and 6 represent the PCR results of C30OExyr1Δace1, which were detected using three pair primers indicated by arrows. d The copy number of xyr1 which was assayed by qPCR using the genomic DNA of RUT-C30, C30OExyr1 and C30OExyr1Δace1 as template, the gene sar1 was represented as single copy

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