Fig. 3

Transcription analysis of C30OExyr1 and C30OExyr1Δace1. a In silico analysis of the putative binding sites of XYR1, ACE1 in the 1000 bp upstream of xylanolytic genes. The promoter sequence was acquired from the T. reesei genomic database. https://genome.jgi.doe.gov/Trire2/Trire2.home.html. The binding sites of XYR1 were searched with GGC(A/T)₃ motif, the strongest binding of GGCTAA and stronger binding of GGCAAA and GGCTAT were additionally indicated. The putative ACE1 binding sites were searched by the AGGCA core sequence. b The relative transcription level of xylanolytic genes. The transcription level of the corresponding gene of RUT-C30 was set as 1, and the transcription level was indicated by log₂(fold change). c The relative transcription level of xyr1 and ace1. The transcription level was measured using the 2^−ΔΔCT method, and the transcription level of sar1 was used for normalization. All the strains were cultured with 3% Avicel, samples at 24 h and 48 h were analyzed for RT-qPCR, and the experiments were conducted with three biological repetitions