Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation

Table 3 The detailed content of enzyme preparation

X/F^a	Cellulase volume (mL)	Total FPase activity (U)	Xylanase volume (mL)	Total xylanase activity (U)	Improvement^b (%)
24	1.56	6.14	0	146.53	100.00
50	1.53	6.07	0.04	315.64	110.82 ± 2.19
100	1.47	5.94	0.11	610.88	114.99 ± 1.63
200	1.36	5.71	0.24	1159.32	126.14 ± 3.91
500	1.07	5.09	0.57	2550.50	134.63 ± 5.37
1000	0.70	4.32	0.99	4321.01	130.58 ± 3.31
1500	0.43	3.75	1.30	5628.10	120.90 ± 4.50
2000	0.23	3.31	1.54	6640.89	111.10 ± 3.27
2770	0	2.83	1.80	7735.83	85.48 ± 7.20

^aX/F means the xylanase activity vs FPase activity in the saccharification system. And equal amount of enzyme (8 mg/g APCS) was loaded for each reaction
^bImprovement refers to the improved glucose yield compared to that using cellulase alone (X/F = 24)

ISSN: 2731-3654