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Table 2 Strains and plasmids used in this study

From: ATP and NADPH engineering of Escherichia coli to improve the production of 4-hydroxyphenylacetic acid using CRISPRi

Name Description Source/purpose
Strain   
E. coli DH5α supE44 Δ(lacZYA-argF) U16980lacZ ΔM15) hsdR17 recA endA1 gyrA96 thi-1 relA1 Invitrogen
E. coli 4HPAA-2 4-Hydroxyphenylacetic acid (4HPAA) producer, E. coli DOPA-30 N, ΔSS9::P37-esaI-P08-esaRI70V-PesaS -PesaR-aro10*-TIGR-feaB*,ΔhpaBC:: P37-esaI-P08-esaRI70V-PesaS -PesaR-aro10*-TIGR-feaB* Lab stock
E. coli 4HPAA-GS-2–4 4HPAA overproducer tolerant to 35.0 g/L 4HPAA with higher 4HPAA titer than E. coli 4HPAA-2 obtained after ARTP mutagenesis and genome shuffling Lab stock
E. coli 4HPAA--∆yahK 4HPAA overproducer, E. coli 4HPAA-GS-2–4, -∆yahK This study
E. coli 4HPAA--∆yahK ∆fecE 4HPAA overproducer, E. coli 4HPAA-GS-2–4,-∆yahK ,∆fecE This study
E. coli 4HPAA-GS-2–4-PesaS-pabA 4HPAA overproducer, E. coli 4HPAA-GS-2–4, PpabA:: P37-esaI-P08-esaRI70V-PesaS This study
E. coli 4HPAA-∆yfp 4HPAA overproducer, E. coli 4HPAA-GS-2–4, -∆yahK , ∆fecE , PpabA:: P37-esaI-P08-esaRI70V-PesaS This study
Plasmid   
pCRISPathBrick* E. coli dcas9 (K848A/K1003A/ R1060A) expression vector [31, 32]
pCas* E. coli cas9 (K848A/K1003A/ R1060A) expression vector [31]
pTargetB E. coli sgRNA expression vector, BglBrick vector [33]