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Table 2 Strains and plasmids used in this study

From: ATP and NADPH engineering of Escherichia coli to improve the production of 4-hydroxyphenylacetic acid using CRISPRi

Name

Description

Source/purpose

Strain

  

E. coli DH5α

supE44 Δ(lacZYA-argF) U16980lacZ ΔM15) hsdR17 recA endA1 gyrA96 thi-1 relA1

Invitrogen

E. coli 4HPAA-2

4-Hydroxyphenylacetic acid (4HPAA) producer, E. coli DOPA-30 N, ΔSS9::P37-esaI-P08-esaRI70V-PesaS -PesaR-aro10*-TIGR-feaB*,ΔhpaBC:: P37-esaI-P08-esaRI70V-PesaS -PesaR-aro10*-TIGR-feaB*

Lab stock

E. coli 4HPAA-GS-2–4

4HPAA overproducer tolerant to 35.0 g/L 4HPAA with higher 4HPAA titer than E. coli 4HPAA-2 obtained after ARTP mutagenesis and genome shuffling

Lab stock

E. coli 4HPAA--∆yahK

4HPAA overproducer, E. coli 4HPAA-GS-2–4, -∆yahK

This study

E. coli 4HPAA--∆yahK ∆fecE

4HPAA overproducer, E. coli 4HPAA-GS-2–4,-∆yahK ,∆fecE

This study

E. coli 4HPAA-GS-2–4-PesaS-pabA

4HPAA overproducer, E. coli 4HPAA-GS-2–4, PpabA:: P37-esaI-P08-esaRI70V-PesaS

This study

E. coli 4HPAA-∆yfp

4HPAA overproducer, E. coli 4HPAA-GS-2–4, -∆yahK , ∆fecE , PpabA:: P37-esaI-P08-esaRI70V-PesaS

This study

Plasmid

  

pCRISPathBrick*

E. coli dcas9 (K848A/K1003A/ R1060A) expression vector

[31, 32]

pCas*

E. coli cas9 (K848A/K1003A/ R1060A) expression vector

[31]

pTargetB

E. coli sgRNA expression vector, BglBrick vector

[33]