|
Strain
| | |
|
E. coli DH5α
|
supE44 Δ(lacZYA-argF) U169 (Φ80lacZ ΔM15) hsdR17 recA endA1 gyrA96 thi-1 relA1
|
Invitrogen
|
|
E. coli 4HPAA-2
|
4-Hydroxyphenylacetic acid (4HPAA) producer, E. coli DOPA-30 N, ΔSS9::P37-esaI-P08-esaRI70V-PesaS -PesaR-aro10*-TIGR-feaB*,ΔhpaBC:: P37-esaI-P08-esaRI70V-PesaS -PesaR-aro10*-TIGR-feaB*
|
Lab stock
|
|
E. coli 4HPAA-GS-2–4
|
4HPAA overproducer tolerant to 35.0 g/L 4HPAA with higher 4HPAA titer than E. coli 4HPAA-2 obtained after ARTP mutagenesis and genome shuffling
|
Lab stock
|
|
E. coli 4HPAA--∆yahK
|
4HPAA overproducer, E. coli 4HPAA-GS-2–4, -∆yahK
|
This study
|
|
E. coli 4HPAA--∆yahK ∆fecE
|
4HPAA overproducer, E. coli 4HPAA-GS-2–4,-∆yahK ,∆fecE
|
This study
|
|
E. coli 4HPAA-GS-2–4-PesaS-pabA
|
4HPAA overproducer, E. coli 4HPAA-GS-2–4, PpabA:: P37-esaI-P08-esaRI70V-PesaS
|
This study
|
|
E. coli 4HPAA-∆yfp
|
4HPAA overproducer, E. coli 4HPAA-GS-2–4, -∆yahK , ∆fecE , PpabA:: P37-esaI-P08-esaRI70V-PesaS
|
This study
|
|
Plasmid
| | |
|
pCRISPathBrick*
|
E. coli dcas9 (K848A/K1003A/ R1060A) expression vector
|
[31, 32]
|
|
pCas*
|
E. coli cas9 (K848A/K1003A/ R1060A) expression vector
|
[31]
|
|
pTargetB
|
E. coli sgRNA expression vector, BglBrick vector
|
[33]
|
