Name | Description | Source/purpose | |
---|---|---|---|
Strain | |||
E. coli DH5α | supE44 Δ(lacZYA-argF) U169 (Φ80lacZ ΔM15) hsdR17 recA endA1 gyrA96 thi-1 relA1 | Invitrogen | |
E. coli 4HPAA-2 | 4-Hydroxyphenylacetic acid (4HPAA) producer, E. coli DOPA-30 N, ΔSS9::P37-esaI-P08-esaRI70V-PesaS -PesaR-aro10*-TIGR-feaB*,ΔhpaBC:: P37-esaI-P08-esaRI70V-PesaS -PesaR-aro10*-TIGR-feaB* | Lab stock | |
E. coli 4HPAA-GS-2–4 | 4HPAA overproducer tolerant to 35.0 g/L 4HPAA with higher 4HPAA titer than E. coli 4HPAA-2 obtained after ARTP mutagenesis and genome shuffling | Lab stock | |
E. coli 4HPAA--∆yahK | 4HPAA overproducer, E. coli 4HPAA-GS-2–4, -∆yahK | This study | |
E. coli 4HPAA--∆yahK ∆fecE | 4HPAA overproducer, E. coli 4HPAA-GS-2–4,-∆yahK ,∆fecE | This study | |
E. coli 4HPAA-GS-2–4-PesaS-pabA | 4HPAA overproducer, E. coli 4HPAA-GS-2–4, PpabA:: P37-esaI-P08-esaRI70V-PesaS | This study | |
E. coli 4HPAA-∆yfp | 4HPAA overproducer, E. coli 4HPAA-GS-2–4, -∆yahK , ∆fecE , PpabA:: P37-esaI-P08-esaRI70V-PesaS | This study | |
Plasmid | |||
pCRISPathBrick* | E. coli dcas9 (K848A/K1003A/ R1060A) expression vector | ||
pCas* | E. coli cas9 (K848A/K1003A/ R1060A) expression vector | [31] | |
pTargetB | E. coli sgRNA expression vector, BglBrick vector | [33] |