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Table 1 Measurement of ATP, ADP, NADH, NAD+, NADPH and NADP+ in strains during exponential growth phase

From: Over-expression of an electron transport protein OmcS provides sufficient NADH for d-lactate production in cyanobacterium

Strains ATP ADP ATP/ADP NADH NAD+ NADH/NAD+ NADPH NADP+ NADPH/NADP+
Syn2973 626.1 ± 24.6 327.1 ± 2.2 1.92 ± 0.09 32.1 ± 2.6 72.6 ± 1.9 0.44 ± 0.05 47.6 ± 4.0 153.3 ± 13.6 0.32 ± 0.05
Syn2973-ΔNbla 605.3 ± 25.7 353.7 ± 23.8 1.71 ± 0.09 39.0 ± 1.0 100.5 ± 2.3 0.39 ± 0.02 50.8 ± 1.1 151.2 ± 7.8 0.34 ± 0.02
Syn2973-OmcS 828.3 ± 13.9 222.6 ± 6.3 3.72 ± 0.04 61.7 ± 2.1 67.4 ± 5.2 0.92 ± 0.10 52.5 ± 1.0 143.1 ± 1.8 0.37 ± 0.01
Syn2973-Ldh 548.1 ± 21.0 296.9 ± 0.9 1.85 ± 0.07 33.7 ± 2.3 100.8 ± 5.8 0.34 ± 0.04 44.7 ± 3.4 134.7 ± 5.0 0.33 ± 0.03
Syn2973-LdhΔGlcD1 541.7 ± 28.0 334.5 ± 15.9 1.62 ± 0.01 27.2 ± 1.4 83.9 ± 2.4 0.32 ± 0.01 40.7 ± 0.4 154.7 ± 0.9 0.26 ± 0.01
Syn2973-LdhOmcS 699.0 ± 12.9 253.4 ± 21.1 2.78 ± 0.28 39.4 ± 1.1 94.0 ± 3.7 0.42 ± 0.01 46.9 ± 0.6 149.0 ± 1.6 0.32 ± 0.01
Syn2973-LdhOmcS-2NP 769.0 ± 19.8 219.9 ± 14.6 3.52 ± 0.32 40.1 ± 2.2 99.7 ± 6.8 0.40 ± 0.01 47.8 ± 4.2 136.8 ± 3.9 0.35 ± 0.04
  1. The data are mean of three independent measurements (mean ± SD; pmol OD730−1)