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Table 4 Methylation sites for R–M systems in newly sequenced Zymomonas strains

From: Zymomonas diversity and potential for biofuel production

Sequence

Type

Strains

Group

% mod

Notes

cCwGg

m4C

CP1

 

99.6

 

cAcnnnnnaTya

m6A

CP1

 

100

 

rgAtcy

m6A

CP3

 

98.6

 

craAnnnnnncTc

m6A

CP3

 

100

 

rcgcAg

m6A

B-23394, B-4492

Z6

 ≥ 99.9

 

gcAnnnnnncTga

m6A

B-23394, B-4492

Z6

100

 

gcAgnnnnnnrta

m6A

CU1, CU1rif2, uvs51

Drainas

100

 

tAynwnnnnctgc

m6A

CU1, CU1rif2, uvs51

Drainas

100

variable in CU1rif2

bgcAnnwnnntgct

m6A

CU1, CU1rif2, uvs51

Drainas

100

different in CU1rif2

agcAnnnnnntgc

m6A

CU1, CU1rif2, uvs51

Drainas

100

variable in uvs51

Ccggtgncar

m4C

PROIMI A1

 

39.1

 

gagntCcnntnnnnnaw

m4C

PROIMI A1

 

92.9

 

cTgcAg

m6A

francensis

 

100

PstI

  1. Capital letters indicate the site of methylation. Methylated bases were detected during sequencing by correlating base incorporation kinetics with base modifications [42]. The methylation motifs were identified using motifMaker software (https://github.com/bioinfomaticsCSU/MultiMotifMaker)