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Table 5 Electroporation and conjugation efficiencies of each strain, relative to ZM4

From: Zymomonas diversity and potential for biofuel production

Group

Strain

Electroporation

Conjugation

ZM4

ZM4

1

1

CP1

CP1

n.d

n.d

B-1960

n.d

7 × 10–2

CP4

CP3

n.d

5 × 10–3

CP4

0.01 ± 0.01

1 × 10–2

Z6

B-23394

n.d

3 × 10–4

Z6

2.15 ± 0.77

9 × 10–3

B-4492

4.78 ± 2.16

1 × 10–3

Drainas

ATCC 10988 T

n.d

1 × 10–3

CU1

n.d

5 × 10–3

CU1 rif2

0.25

4 × 10–4

uvs51

n.d

2 × 10–2

None

PROIMI A1

n.d

2 × 10–3

francensis

n.d

n.d

pomaceae

n.d

n.d

  1. Efficiency of electroporation for ZM4 was 15 CFU per 1 µg of pRL814 DNA. Efficiency of conjugation was 1.25 × 10–4 calculated as number of spectinomycin-resistant colonies divided by number of colonies on plates without spectinomycin
  2. n.d. not detected