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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: From simple and specific zymographic detections to the annotation of a fungus Daldinia caldariorum D263 that encodes a wide range of highly bioactive cellulolytic enzymes

Fig. 4

SDS-PAGE can be used for detecting exoglucanase activity on MUC zymography. a MUC zymography can also reveal cellulase activities on SDS-PAGE gels. b Addition of 2% glucose in MUC zymography represses BGL activity on SDS-PAGE gel. N188, C1.5L, TRX and D2-BGL were used as positive and negative controls of glucose suppression of BGL activity. c Coomassie Blue staining shows protein samples loaded into SDS-PAGE gel. The protein amount used was C1.5L, 2600 ng; N188, 150 ng; D263, 340 ng; TRX, 1100 ng; D2-BGL, 1000 ng. All experiments were conducted on 8% SDS-PAGE gels with at least three biological replicates

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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