Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

Table 1 Details of the bioprocesses from Avicel to d-glucaric acid

	SHF^a	SSF^b	CBP^c	Glu^d	G + I^e
Substrate	Avicel	Avicel	Avicel	Glucose	Glucose and myo-inositol
Avicel for bioprocesses (g)	50	50	15	0	0
Glucose (g)	28.31	N.A	N.A	30	30
myo-Inositol (g)	0	0	0	0	10.8
Cellulase dosage (FPIU/g glucan)	25	25	0		0
Total cellulase loading (FPIU)	1250	1250	0	0	0
Avicel for cellulase production (g)	13.07	13.07	0	0	0
Total Avicel (g)	63.07	63.07	15	0	0
d-Glucaric acid (g)	3.74	1.22	0.54	4.52	9.53
Yield (g d-glucaric acid/g total Avicel)	0.0593	0.0193	0.0360	N.A	N.A

Glu, glucose
G + I, glucose and myo-inositol
N.A., not applicable
^aSHF, separated saccharification and fermentation by S. cerevisiae LGA-1
^bSSF, simultaneous saccharification and fermentation by S. cerevisiae LGA-1
^cCBP, consolidated bioprocessing by T. reesei Rut-C30 and S. cerevisiae LGA-1
^dFed-batch fermentation of glucose by S. cerevisiae LGA-1
^eFed-batch fermentation of glucose and myo-inositol by S. cerevisiae LGA-1

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