Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

Table 2 Details of the bioprocesses from steam-exploded corn stover (SECS) to d-glucaric acid

	SHF^a	SSF^b	CBP^c	Glu^d	G + I^e
Substrate	SECS	SECS	SECS	Glucose	Glucose and myo-inositol
SECS for bioprocesses (g)	100	100	15	0
Glucose (g)	39.73	N.A	N.A	30	30
myo-Inositol (g)	0	0	0	0	10.8
Cellulase dosage (FPIU/g glucan)	25	25	0	0	0
Total cellulase loading (FPIU)	2500	2500	0	0	0
SECS for cellulase production (g)	30.61	30.61	0	0	0
Total SECS (g)	130.61	130.61	15	0	0
d-Glucaric acid (g)	4.92	1.14	0.45	4.52	9.53
Yield (g d-glucaric acid/g total SECS)	0.0377	0.0087	0.0300	N.A	N.A

Glu, glucose
G + I, glucose and myo-inositol
N.A., not applicable
^aSHF, separated saccharification and fermentation by S. cerevisiae LGA-1
^bSSF, simultaneous saccharification and fermentation by S. cerevisiae LGA-1
^cCBP, consolidated bioprocessing by T. reesei Rut-C30 and S. cerevisiae LGA-1
^dFed-batch fermentation of glucose by S. cerevisiae LGA-1
^eFed-batch fermentation of glucose and myo-inositol by S. cerevisiae LGA-1

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