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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Increasing lipid yield in Yarrowia lipolytica through phosphoketolase and phosphotransacetylase expression in a phosphofructokinase deletion strain

Fig. 2

source organisms codon-optimized to S. cerevisiae (GeneArt) were expressed in YB-392 under the control of the Y. lipolytica EXP1 promoter using linear integrating expression cassettes. Cell-free extracts (CFE) from four transformants per test gene were analyzed for Pta activity using a DTNB assay. Data are presented as fold change of the measured specific activity over the averaged specific activity of the parent strain YB-392 (dashed line), which is included as control

Heterologous Pta activity in Y. lipolytica. PTA genes from seven different

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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