Fig. 3

source organisms codon-optimized to Y. lipolytica (ATGme [59]) were expressed in YB-392 under the control of the Y. lipolytica TEF1 promoter on replicating plasmids. Cell-free extracts from 4 transformants per test gene were analyzed by ferric hydroxamate assay to measure Xpk activity on ribose 5-phosphate (R5P, black bars) and fructose 6-phosphate (F6P, grey bars). Absorbance was measured at 540 nm and normalized to total protein in the crude cell-free extract after a reaction time of 30 min. Data are presented as fold change of the normalized absorbance over the averaged normalized absorbance of the parent strain YB-392 (dashed line), which is included as control