Fig. 5
source and no nitrogen to induce lipid production
Engineering the Xpk/Pta pathway in a Δpfk1 Y. lipolytica strain. a Pta and Xpk activity. Pta activity in all strains shown was measured using the DTNB assay (black bars). Xpk activity in the control strain YB-392 and all the strains that obtained a copy of CaXPK through transformation (NS1281, NS1292, NS1322, NS1457 and NS1475) was measured using the ferric hydroxamate assay with ribose 5-phosphate as the substrate (grey bars). NS1047, NS1341, NS1352 and NS1420 were excluded from this assay. b Growth and lipid accumulation assays. OD600 was measured after 2 days of growth in lipid production media (black bars). Lipid accumulation was measured as fluorescence/OD after overnight growth in glycerol followed by seven days of culture in modified Verduyn media (grey bars). Modified Verduyn media contained glucose as the only carbon