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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Identification and characterization of proteins of unknown function (PUFs) in Clostridium thermocellum DSM 1313 strains as potential genetic engineering targets

Fig. 7

a Western blot of WP_003519433.1 expression in E. coli. L: protein ladder; C: protein purified from no IPTG-induced cells (negative control); Lane 1: protein purified from cells induced with 0.1 mM IPTG; and 2: protein purified from cells induced with 1 mM IPTG. The band signals observed in lanes 1 and 2 in the red box confirmed the identity of WP_003519433.1 with an expected protein size of 50.4 kDa. b Total ion chromatography of high cell density E. coli whole-cell conversion of 2-phenylethyl alcohol. E. coli harboring empty plasmid was used a negative control. c Mass-to-charge ratio of the selected 2-phenylethyl acetate peak. Here, the eluted peaks at the retention of 15.5 min in panel B confirmed that 2-phenylethyl acetate was produced by Ec1074 carrying WP_003519433.1 with the expected mass fragmentation shown in panel C

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