Fig. 3
a Fluorescence intensity of recombinant E. limosum strains during growth in presence of the fluorogen TFLime. Cells were cultivated using 30 mM glucose. Monitored were OD600 and fluorescence intensity of E. limosum [pMTL83251_PbgaL_FAST] with induced and non-induced gene expression as well as E. limosum [pMTL83251]. b Micrographs of E. limosum [pMTL83251_PbgaL_FAST] and E. limosum [pMTL83251] after 72 h of incubation. Gene expression of cells was either induced or non-induced. c Density plots of E. limosum [pMTL83251_PbgaL_FAST] and E. limosum [pMTL83251] after 72 h of incubation. Gene expression of cells was either induced or non-induced. d Number of fluorescent E. limosum cells determined after 24 h, 48 h, and 72 h cultivation. n = 3