Fig. 4

Fluorescence intensity of recombinant E. limosum strains grown on methanol (100 mM) in presence of the fluorogen ^TFLime. a OD₆₀₀ and fluorescence intensity of E. limosum [pMTL83251], b E. limosum [pMTL83251_P_thlA_FAST], c E. limosum [pMTL83251_P_bgaL_FAST], and d E. limosum [pMTL83251_P_bgaL_tdFAST2] were monitored. Gene expression of E. limosum [pMTL83251_P_bgaL_FAST] and E. limosum [pMTL83251_P_bgaL_tdFAST2] was either induced by lactose or non-induced. Time of induction with lactose is indicated with the vertical dotted line. n=3, error bars show standard deviation. e Genetic maps of plasmids representing pMTL83251_P_thlA_FAST (top), pMTL83251_P_bgaL_FAST (middle), and pMTL83251_P_bgaL_tdFAST2 (bottom). Fluorescence during growth of cells harboring respective plasmids are shown in panel b, c, and d, respectively. f Maximal fluorescence intensity of recombinant E. limosum strains in the presence of the fluorogen ^TFLime or ^TFCoral. Mean fluorescence was determined during stationary growth phase at an OD₆₀₀ of ~ 1.5, n = 3, error bars show standard deviation. n = 3