Fig. 5From: Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosumGrowth experiment with E. limosum [pMTL83251_PbgaL_C-FAST-AdhE2] and E. limosum [pMTL83251_PbgaL_N-FAST-AdhE2]. Cells were cultivated using a 30 mM glucose or b 100 mM methanol. Gene expression of cells was either induced by lactose or non-induced. Time of induction with lactose is indicated with the vertical dotted line. OD600, fluorescence intensity in presence of TFLime, glucose or methanol consumption as well as acetate, butyrate, ethanol, and butanol production were monitored. Error bars indicate standard deviations. n = 3Back to article page