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Table 6 Plasmids used in this study

From: Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum

Plasmid Description Source
pMTL83251 ColE1 ori-, pCB102 ori+, Emr, traJ, lacZ [83]
pMTL83151_gusA_PbgaL ColE1 ori-, pCB102 ori+, Cmr, traJ, lacZ, gusA from E. coli, bgaR-PbgaL from C. perfringens [49]
pJIR750_act pJIR750; Pthl, thlA (CAC2873), ctfA/B (CA_P0163/P0164), adc (CA_P0165) from C. acetobutylicum [18]
p95thlsupFAST ColE1 ori-, repL ori+, Ampr, Emr, lacZ, Pthlsup, feg [31]
pMTL83251_Pthlsup_FAST pMTL83251; Pthlsup, feg from p95thlsupFAST This work
pMTL83251_PthlA_FAST pMTL83251; PthlA, from pMTL83251_PthlA_act; feg from p95thlsupFAST This work
pMTL83251_PbgaL_FAST pMTL83251; bgaR-PbgaL from C. perfringens; feg from p95thlsupFAST This work
pEX-A128_tdFAST2-opt pUC ori-, Ampr, MSC, gene encoding tdFAST2 codon optimized for E. limosum Eurofins Genomics Germany GmbH, Ebersberg, Germany
pMTL83251_PbgaL_tdFAST2 pMTL83251; bgaR-PbgaL from C. perfringens; gene encoding tdFAST2 codon optimized for E. limosum This work
pMTL83251_PbgaL_AdhE2 pMTL83251; bgaR-PbgaL from C. perfringens; adhE2 from C. acetobutylicum This work
pMTL83251_PbgaL_C-FAST-AdhE2 pMTL83251; bgaR-PbgaL from C. perfringens; adhE2 from C. acetobutylicum C-terminal tagged with feg from p95thlsupFAST This work
pMTL83251_PbgaL_N-FAST-AdhE2 pMTL83251; bgaR-PbgaL from C. perfringens; adhE2 from C. acetobutylicum N-terminal tagged with feg from p95thlsupFAST This work
pMTL83251_PthlA_act pMTL83251; Pthl, thlA, ctfA/B, adc from pJIR_act This work
pMTL83251_PthlA_C-FAST-Adc pMTL83251; PthlA, thlA, ctfA/B, from pJIR_act; adc from C. acetobutylicum C-terminal tagged with feg from p95thlsupFAST This work
pMTL83251_PthlA_N-FAST-Adc pMTL83251; PthlA, thlA, ctfA/B, from pJIR_act; adc from C. acetobutylicum N-terminal tagged with feg from p95thlsupFAST This work