Table 6 Plasmids used in this study

pMTL83251

ColE1 ori^-, pCB102 ori⁺, Em^r, traJ, lacZ

[83]

pMTL83151_gusA_P_bgaL

ColE1 ori^-, pCB102 ori⁺, Cm^r, traJ, lacZ, gusA from E. coli, bgaR-P_bgaL from C. perfringens

[49]

pJIR750_act

pJIR750; P_thl, thlA (CAC2873), ctfA/B (CA_P0163/P0164), adc (CA_P0165) from C. acetobutylicum

[18]

p95thl^supFAST

ColE1 ori^-, repL ori⁺, Amp^r, Em^r, lacZ, P_thl^sup, feg

[31]

pMTL83251_P_thl^sup_FAST

pMTL83251; P_thl^sup, feg from p95thl^supFAST

This work

pMTL83251_P_thlA_FAST

pMTL83251; P_thlA, from pMTL83251_P_thlA_act; feg from p95thl^supFAST

This work

pMTL83251_P_bgaL_FAST

pMTL83251; bgaR-P_bgaL from C. perfringens; feg from p95thl^supFAST

This work

pEX-A128_tdFAST2-opt

pUC ori^-, Amp^r, MSC, gene encoding tdFAST2 codon optimized for E. limosum

Eurofins Genomics Germany GmbH, Ebersberg, Germany

pMTL83251_P_bgaL_tdFAST2

pMTL83251; bgaR-P_bgaL from C. perfringens; gene encoding tdFAST2 codon optimized for E. limosum

This work

pMTL83251_P_bgaL_AdhE2

pMTL83251; bgaR-P_bgaL from C. perfringens; adhE2 from C. acetobutylicum

This work

pMTL83251_P_bgaL_C-FAST-AdhE2

pMTL83251; bgaR-P_bgaL from C. perfringens; adhE2 from C. acetobutylicum C-terminal tagged with feg from p95thl^supFAST

This work

pMTL83251_P_bgaL_N-FAST-AdhE2

pMTL83251; bgaR-P_bgaL from C. perfringens; adhE2 from C. acetobutylicum N-terminal tagged with feg from p95thl^supFAST

This work

pMTL83251_P_thlA_act

pMTL83251; P_thl, thlA, ctfA/B, adc from pJIR_act

This work

pMTL83251_P_thlA_C-FAST-Adc

pMTL83251; P_thlA, thlA, ctfA/B, from pJIR_act; adc from C. acetobutylicum C-terminal tagged with feg from p95thl^supFAST

This work

pMTL83251_P_thlA_N-FAST-Adc

pMTL83251; P_thlA, thlA, ctfA/B, from pJIR_act; adc from C. acetobutylicum N-terminal tagged with feg from p95thl^supFAST

This work