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Table 7 Primers used in this study

From: Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum

Primer

Sequence 5′–3′

Length (bp)

FW_C1_adhE2

ttaaatgtattgggagggtggatccatgaaagttacaaatcaaaaagaac

50

RV_N2_adhE2

agcttgcatgtctgcaggcctcgagttaaaatgattttatatagatatccttaagttc

58

FW_act_NdeI

gaccgcggccgctgtatccatatgtcaagaagaggcac

38

RV_act_XhoI

agcttgcatgtctgcaggcctcgagttacttaagataatcatatataacttcag

54

FW_Pthlsup_FAST_NdeI

atgaccgcggccgctgtatccatatgtttttaacaaaaagtattgaaatttg

52

RV_Pthlsup_FAST_XhoI

aagcttgcatgtctgcaggcctcgagtcataccctcttaac

41

FW_PbgaL_NdeI

gaccgcggccgctgtatccatatgtaatttagatattaattctaaattaagtgaaattaatatag

65

RV_PbgaL_BamHI

caaatgctacgtgttccatggatccaccctcccaatacatttaaaataattatg

54

FW_Pthl_NdeI

gaccgcggccgctgtatccatatgtcaagaagaggcacctcatc

44

RV_Pthl_BamHI

caaatgctacgtgttccatggatcctaacctcctaaattttgatacgg

48

FW_tdFAST2-opt_BamHI

ttaaatgtattgggagggtggatccatggagcatgtagcttttg

44

RV_tdFAST2-opt_XhoI

agcttgcatgtctgcaggcctcgagtcaaacccgtttgacgaag

44

RV_C1_adhE2-FAST

ctacgtgttcagaaccaccaccaccaaatgattttatatagatatccttaagttc

55

FW_C2_adhE2-FAST

aaaatcatttggtggtggtggttctgaacacgtagcatttggaag

45

RV_C2_FAST

agcttgcatgtctgcaggcctcgagtcataccctcttaacgaaaac

46

FW_N1_FAST

ttaaatgtattgggagggtggatccatggaacacgtagcatttg

44

RV_N1_FAST-adhE2

ttgtaactttagaaccaccaccacctaccctcttaacgaaaac

43

FW_N2_FAST-adhE2

taagagggtaggtggtggtggttctaaagttacaaatcaaaaagaactaaaac

53

FW_C1_adc

acccatggctgtttaggtaccttttatgttaaaggatgaagtaattaaac

50

RV_C1_adc-FAST

ctacgtgttcagaaccaccaccacccttaagataatcatatataacttcagc

52

FW_C2_adc-FAST

ttatcttaagggtggtggtggttctgaacacgtagcatttggaag

45

FW_N1_FAST-adc

acccatggctgtttaggtaccttttatggaacacgtagcatttg

44

RV_N1_FAST-adc

catcctttaaagaaccaccaccacctaccctcttaacgaaaac

43

FW_N2_FAST-adc

taagagggtaggtggtggtggttctttaaaggatgaagtaattaaacaaattag

54

RV_N2_adc

agcttgcatgtctgcaggcctcgagttacttaagataatcatatataacttcag

54