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Table 4 Summary of protein changes in each strain during salt stress

From: Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources

Protein accession ID Protein name Pathway/role Salt stress treatment comparisons with relative protein changes (values indicate fold change)
    C. reinhardtii C. nivalis
    0–11 h salt 11–18 h salt 0–18 h salt 18 h control–18 h salt 0–80 h salt 80–168 h salt 0–168 h salt 168 h control–168 h salt
Stress proteins
A8HYV3/ Q39603 Heat shock protein 70B Chloroplast localised heat shock protein protecting against photoinhibition [84] ↑ 1.073  →  ↑ 1.077  →  ↓ -1.340  →  ↓ -1.363 ↓ -1.708
A8J3P5 Heat shock protein 70E Cytosolic heat shock protein [84]  →   →   →  ↓ -1.172  →   →   →   → 
A8JEU4 Heat shock protein 70A Cytosolic heat shock protein [84]  →   →   →   →  ↓ -1.358  →  ↓ -1.334 ↓ -1.880
A8IZU0 Heat shock protein 70C Mitochondrial heat shock protein [84]  →   →   →   →  ↓ -1.336  →  ↓ -1.386 ↓ -1.731
A8J1U1 Heat shock protein 90A Cytosolic heat shock protein [85]  →   →   →   →   →   →  ↓ -1.189 ↓ -1.848
Q66T67 Heat shock protein 90C Chloroplastic heat shock protein [85]  →   →   →   →   →   →  ↓ -1.543 ↓ -1.868
A8I7T1 Heat shock protein 90B (Fragment) ER localised heat shock protein [86]  →   →   →   →   →   →   →  ↓ -1.771
Q1RS84 Aldehyde-alcohol dehydrogenase Aldehyde scavenging (during lipid peroxidation) [42] ↑ 1.134  →  ↑ 1.106  →   →   →   →   → 
A8JHR9/ A8JHS0/ P49644 Glyceraldehyde-3-phosphate dehydrogenase Glycolysis (and carbohydrate metabolism)  →  ↑ 1.155 ↑ 1.284  →  ↑ 2.166  →  ↑ 2.200  → 
A8HXA6 Glutathione reductase Reduction of glutathione disulphide [45] ↓ -1.195 ↑ 1.201  →   →   →  ↑ 1.277  →   → 
A8JG06 Programmed cell death protein 6-interacting protein Protein self-association and ubiquitin binding  →   →   →   →   →  ↑ 1.208  →   → 
A8J1C1 Ubiquitin-activating enzyme E1 Ubiquitin conjugation cascade ↓ -1.088 ↑ 1.169  →   →   →  ↑ 1.254 ↑ 1.198 ↑ 1.097
A8J5Z8 Aldehyde dehydrogenase Aldehyde detoxification, ROS scavenging, inhibits lipid peroxidation  →  ↑ 1.180 ↑ 1.274 ↑ 1.271  →   →  ↑ 1.306  → 
A8JB85 Autophagy-related protein Autophagy ↑ 1.385  →  ↑ 1.274  →   →   →  ↑ 1.352  → 
A8J431 Stress-related chlorophyll a/b binding protein 2 Non-photochemical quenching, protect photosynthetic apparatus from photo-oxidative damage  →   →   →  ↑ 1.776  →   →  ↑ 1.572  → 
Photosynthesis proteins
Q39588 Carbonic anhydrase, alpha type Carbon dioxide fixation [87]  →   →  ↓ -1.202 ↓ -1.135  →   →   →   → 
Q8S567 Chlorophyll a/b-binding protein Photosynthesis and light harvesting ↓ -1.190  →  ↓ -1.191  →   →   →   →   → 
A8J270 Chlorophyll a-b binding protein of LHCII Light harvesting in photosystem I  →   →   →  ↑ 1.144  →   →   →   → 
Q8S3T9 Chlorophyll a-b binding protein of LHCII Light harvesting in photosystem I  →   →   →  ↑ 1.171  →   →   →   → 
A8HP84/ P50362 Glyceraldehyde-3-phosphate dehydrogenase Photosynthesis, Calvin cycle  →   →   →   →  ↓ -1.529  →  ↓ -1.682 ↓ -2.147
Q75VY7 Light-harvesting chlorophyll-a/b protein of photosystem I Chlorophyll binding, light harvesting in photosystem I ↓ -1.240  →   →  ↓ -1.347  →   →   →   → 
A8ITV3 Light-harvesting protein of photosystem I Chlorophyll binding, light harvesting in photosystem I ↓ -1.190  →  ↓ -1.191  →   →   →   →   → 
Q93VE0 Light-harvesting chlorophyll-a/b binding protein LhcII-4 Chlorophyll binding, light harvesting in photosystem I  →   →   →  ↑ 1.147  →   →   →   → 
A8J0B1 Low-CO2-inducible protein Inorganic carbon uptake [52] ↓ -1.161  →   →   →   →   →   →   → 
A8J225 Low-CO2-inducible protein (Fragment) Inorganic carbon uptake [52] ↑ 1.285  →  ↑ 1.240 ↑ 1.385  →   →   →   → 
Q75NZ1 Low-CO2 inducible protein Inorganic carbon uptake [52]  →  ↑ 1.067  →   →   →   →   →   → 
A8IT08 Low-CO2-inducible chloroplast envelope protein L-orthinine transmembrane transport activity  →   →  ↑ 1.268 ↑ 1.390  →   →   →   → 
A8JEV1 Oxygen evolving enhancer protein 3 Photosynthetic electron transfer chain, calcium ion binding  →  ↓ -1.150 ↓ -1.141 ↓ -1.226 ↓ -1.495  →  ↓ -1.674  → 
A8J0E4 Oxygen-evolving enhancer protein 1 of photosystem II Photosystem II assembly and stability, oxygen evolving activity  →  ↓ -1.094 ↓ -1.145 ↓ -1.290 ↓ -1.864  →  ↓ -2.186 ↓ -2.300
A8IYH9 Oxygen-evolving enhancer protein 2 of photosystem II Photosynthesis, calcium ion binding  →   →  ↓ -1.136 ↓ -1.108 ↓ -1.520  →  ↓ -1.626 ↓ -2.072
P12852 Oxygen-evolving enhancer protein 3, chloroplastic Photosynthesis, calcium ion binding  →  ↓ -1.150 ↓ -1.141 ↓ -1.226 ↓ -1.495  →  ↓ -1.674  → 
Q00914 Photosystem I iron-sulphur centre Photosynthetic electron transport in photosystem I  →   →   →   →  ↓ -1.672  →  ↓ -1.932  → 
P13352 Photosystem I reaction centre subunit VI, chloroplastic Photosynthesis  →   →   →   →   →   →   →  ↓ -2.285
Q5NKW4 Photosystem I reaction centre subunit II, 20 kDa Photosynthesis  →  ↓ -1.177  →   →   →   →   →   → 
Q39615 Photosystem I reaction centre subunit II, chloroplastic Photosynthesis  →  ↓ -1.177  →   →   →   →   →   → 
P12154 Photosystem I P700 chlorophyll a apoprotein A1 Photosynthesis, electron transfer, chlorophyll binding  →   →  ↓ -1.261  →  ↓ -1.823  →  ↓ -1.864  → 
P09144 Photosystem I P700 chlorophyll a apoprotein A2 Photosynthesis, electron transfer, chlorophyll binding  →   →  ↓ -1.200  →  ↓ -1.643  →  ↓ -1.614  → 
P10898 Photosystem II CP43 reaction centre protein Photosynthetic electron transport in photosystem II, chlorophyll binding  →   →   →   →  ↓ -1.623  →  ↓ -1.824 ↓ -1.469
P37255 Photosystem II CP47 reaction centre protein Photosynthetic electron transport in photosystem II, chlorophyll binding  →   →   →   →  ↓ -1.583  →  ↓ -1.693  → 
P06007 Photosystem II D2 protein Photosynthetic electron transport in photosystem II, chlorophyll binding  →   →   →   →  ↓ -1.611  →  ↓ -1.634  → 
P07753 Photosystem II protein D1 Photosynthetic electron transport in photosystem II, chlorophyll binding  →   →   →   →  ↓ -1.840  →  ↓ -1.727 ↓ -1.445
A8J4S1 Photosystem I reaction centre subunit III Photosynthesis, protein domain specific binding  →  ↓ -1.255 ↓ -1.289 ↓ -1.461  →   →   →   → 
P12356 Photosystem I reaction centre subunit III, chloroplastic Photosynthesis, protein domain specific binding  →  ↓ -1.255 ↓ -1.289 ↓ -1.461  →   →   →   → 
A8HVJ9 Photosystem II stability/assembly factor HCF136 fragment Photosystem II biogenesis  →   →   →  ↓ -1.190  →   →   →   → 
A8JGP9 Ribulose bisphosphate carboxylase small chain Carbon fixation, photorespiration, photosynthesis ↓ -1.213  →  ↓ -1.356  →   →   →   →   → 
P00873 Ribulose bisphosphate carboxylase small chain 1, chloroplastic Carbon fixation, photorespiration, photosynthesis ↓ -1.213  →  ↓ -1.356  →   →   →   →   → 
P08475 Ribulose bisphosphate carboxylase small chain 2, chloroplastic Carbon fixation, photorespiration, photosynthesis ↓ -1.219  →  ↓ -1.357  →   →   →   →   → 
P00877 Ribulose bisphosphate carboxylase large chain Photorespiration, reductive pentose-phosphate cycle  →  ↓ -1.078 ↓ -1.154 ↓ -1.110 ↓ -1.810  →  ↓ -1.991 ↓ -2.751
P23489 Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic Photosynthesis regulation  →  ↓ -1.107 ↓ -1.108 ↓ -1.186  →   →  ↓ -1.555 ↓ -2.809
Q6SA05 Rubisco activase Photosynthesis regulation  →  ↓ -1.107 ↓ -1.108  →   →   →  ↓ -1.555 ↓ -2.809
Carbohydrate proteins
Q2VA40 Alpha-1,4 glucan phosphorylase Starch degradation [88] ↑ 1.123 ↑ 1.105 ↑ 1.240 ↑ 1.122 ↑ 1.237 ↑ 1.158 ↑ 1.433 ↓ -1.688
Q2VA41/ A8IYK1 Alpha-1,4 glucan phosphorylase Starch degradation [88] ↑ 1.096  →  ↑ 1.147  →   →   →   →   → 
A2PZC2 UDP-glucose:protein transglucosylase Polysaccharide biosynthesis  →   →   →   →  ↓ -1.535  →  ↓ -1.713 ↓ -2.510
A8HW52 Starch branching enzyme Starch biosynthesis  →   →   →   →   →   →   →  ↓ -2.318
A8IHX1 Starch branching enzyme Starch biosynthesis  →   →  ↑ 1.207  →   →   →   →   → 
A8J914 UDP-glucose 6-dehydrogenase Starch catabolism for glycolysis [58] ↑ 1.263 ↑ 1.239 ↑ 1.562 ↑ 1.478  →   →   →   → 
A2PZC3 UDP-glucose 6-dehydrogenase Starch catabolism for glycolysis [58] ↑ 1.298 ↑ 1.231 ↑ 1.595 ↑ 1.461  →   →   →  ↑ 2.008
Lipid proteins
A8HNN4/ A0A0B5KTL4 Glycerol-3-phosphate dehydrogenase Glycerol metabolism, link between carbohydrate and lipid metabolism ↑ 1.675 ↑ 1.169 ↑ 1.959 ↑ 1.959 ↑ 2.135 ↑ 1.192 ↑ 2.544 ↑ 2.648
A0A0B5KYA7 Glycerol-3-phosphate dehydrogenase Glycerol metabolism, link between carbohydrate and lipid metabolism ↑ 1.719 ↑ 1.179 ↑ 2.027 ↑ 2.064  →   →   →   → 
A8HNN6 Glycerol-3-phosphate dehydrogenase (fragment) Glycerol metabolism, link between carbohydrate and lipid metabolism ↑ 1.744 ↑ 1.189 ↑ 2.073 ↑ 2.109  →   →   →   → 
A8J6J6 Acetyl-CoA acyltransferase Catabolic beta-oxidation of fatty acids [63] ↑ 1.162  →  ↑ 1.181 ↑ 1.209  →   →  ↑ 1.250 ↑ 1.375
A8J2S0 Citrate synthase Tricarboxylic acid cycle  →   →   →   →   →   →   →  ↓ -2.725
Q6DN05 Betaine lipid synthase Betaine lipid synthesis, including DGTS synthesis  →  ↓ -1.093  →   →   →   →   →   → 
A8HXT4 Pyruvate carboxylase Pyruvate metabolism, gluconeogenesis, links carbohydrate and lipid metabolism  →  ↓ -1.047 ↓ -1.100  →   →   →   →   → 
A8JGF4 Biotin carboxylase, acetyl-CoA carboxylase component Malonyl-CoA biosynthesis, lipid metabolism  →   →   →  ↓ -1.113 ↓ -1.479  →  ↓ -1.482 ↓ -1.860
Q763T6 UDP-sulfoquinovose synthase Glycerolipid metabolism, sulfoquinovosyl diacylglycerol (SQDG) metabolism  →   →   →   →  ↓ -1.275  →  ↓ -1.289 ↓ -1.770
A8J1T4 Dihydrolipoyl dehydrogenase Acetyl CoA production, cell redox homeostasis  →   →   →   →  ↑ 1.110  →   →  ↑ 1.149
  1. Changes in stress-related proteins, photosynthesis-related proteins, carbohydrate-related proteins and lipid-related proteins are shown for C. reinhardtii and C. nivalis subjected to salt stress. Arrows indicate up-regulation (↑), down-regulation (↓) and not significantly changed ( →). Values indicate fold changes