Fig. 7
H2O2 production curve (A) and HPEAC-PAD profile (B) to confirm the functional expression of AoAA13 and AfAA11B by using either the pBSYPGCW14Z or the pBSYPGCW14Z-OST1 expression plasmid. A For the H2O2 production curve 3 µM AoAA13/CuSO4, 100 µM Amplex Red and 0.025 mg/ml HRP were used, and the reaction was initiated by the addition of 50 µM AscA (all final concentrations). Control reactions did not contain catalyst. The presented curves represent the average of three independent replicates. B To assess the substrate oxidation ability of AfAA11B standard aerobic reactions (green) with 1 µM LPMO, 1 mM (GlcNAc)4 in the presence (solid lines) and absence (dashed lines) of 1 mM AscA were made. To show that peaks correspond to oxidized products, a standard containing 150 µM oxidized chitobiose and chitotriose is shown (grey)