Enzyme | Plasmid + SP | Supernatant | Fivefold concentrated | Purified (obtained from 50 ml cultivation supernatant) | Recovered protein | |||
---|---|---|---|---|---|---|---|---|
mg | mg/ml | mg | mg/ml | mg | mg/ml | % | ||
LsAA9A | pBSY3Z + native | 21 ± 5 | 0.042 ± 0.010 | 22.5 ± 0.4 | 0.225 ± 0.004 | 0.61 ± 0.01 | 1.2 ± 0.02 | 27 |
pBSYPGCW14Z + native | 28 ± 2 | 0.056 ± 0.004 | 24.7 ± 1.7 | 0.247 ± 0.017 | 0.6 ± 0.03 | 1.4 ± 0.05 | 24 | |
NcAA9C | pBSY3Z + native | 22.5 ± 1.5 | 0.045 ± 0.003 | 20.7 ± 2.3 | 0.207 ± 0.023 | 0.39 ± 0.02 | 0.8 ± 0.05 | 18 |
pBSYPGCW14Z + native | 76 ± 7 | 0.153 ± 0.014 | 70.5 ± 0.5 | 0.705 ± 0.005 | 2.08 ± 0.06 | 2.9 ± 0.03 | 29 |