Novel molecular biological tools for the efficient expression of fungal lytic polysaccharide monooxygenases in Pichia pastoris

Table 2 Comparison of expression constructs, cultivation and purification methods and the thereof resulting yields of different LPMO production protocols found in literature

Enzyme	Plasmid	Promoter	Signal peptide	Cultivation method	Purification steps	Total protein per liter culture media prior purification	Total protein per liter culture media after purification	Reference
TrCel61A	pPpT4	AOX1	Native	2-L fed-batch reactor	N.A	> 400 mg	N.A	[18]
NcAA9J	pPICZαA	AOX1	Native	7-L fed-batch reactor	Three chromatographic steps	1574 mg^a	24 mg^a	[22]
NcAA9C	pPICZαA	AOX1	Native	7-L fed-batch reactor	Two chromatographic steps	2762 mg	67 mg^a	[22]
NcAA9F	pPICZαA	AOX1	Native	7-L fed-batch reactor	Three chromatographic steps	1818 mg^a	2.4 mg^a	[22]
NcAA9E	pPICZαA	AOX1	Native	7-L fed-batch reactor	Three chromatographic steps	1327 mg^a	2.2 mg^a	[22]
NcAA9C	pPICZαA	AOX1	Native	500-mL fed-batch reactor	Two chromatographic steps	1370 mg^a	90 mg^a	[29]
AaAA16	pPICZαA	AOX1	Native	1.3-L fed-batch reactor	IMAC^b, IEC	N.A	500 mg after IMAC	[30]
Several AA9s	pPICZT	AOX1	Native	2-L shake flask	IMAC	34 mg per 100 g cell wet weight	N.A	[31]

ISSN: 2731-3654