Skip to main content
Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution

Fig. 2

Sequence and structure analyses of D2-BGL and Aspergillus GH3 β-glucosidases. a Alignment between D2-BGL (CrD2-BGL), Trichoderma reesei BGL (TrBGL), A. aculeatus BGL1 (AaBGL1), A. niger BGL1 (AnBGL1) and A. niger ASKU28 BGL (AnASKU). Mutation points discovered in D2-BGL and Aspergillus enzymes are highlighted in yellow or green, respectively. b Structure superposition between D2-BGL (PDB: 6JXG, in green) and AaBGL1 (PDB: 4IIH, in grey) at the active site. F256 and Y260 in D2-BGL are presented in red stick, and Q201 in AaBGL1 is presented in black stick. TBC: thiocellobiose

Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us