Fig. 5

Carbohydrate esterase (CE) activity of BD-FAE. A 1 µg BD-FAE on 1 mM pNP-ferulate at pH 7 and 40 °C, n = 2. B 1.5% and 4.5% (g enzyme / g dry matter substrate) BD-FAE on acetylated glucuronoxylan (AcGX, 15 mg/mL final concentration) at pH 6.0 and 40 °C analyzed with acetic acid kit (K-ACETRM, enzyme and buffer blanks subtracted, see Additional file 2: Table S4), n = 2 and C MALDI-TOF spectra before and after incubating 3% (g enzyme / g dry matter substrate) BD-FAE on 10 mg/mL acetylated and feruloylated xylooligosaccharides from corn fiber (AcFaXOS) at pH 7.0 and 40 °C for 4 h (n = 2). All m/z were sodium adducts. Structural annotation is based on [11]. P pentose, H hexose, Ac acetyl, Fa feruloyl, orange star d-xylosyl, green star l-arabinosyl, yellow circle l-galactosyl residues