Fig. 9
The oxidative activity and decolorizing rate of Il-DyP4 and the W380 variants. a–c The oxidative activity for DMP, guaiacol and ABTS, respectively. d–i The decolorizing rate for RB 4, RB 5, RB 19, 5B, MO and RV 5, respectively. The assay mixtures (1 mL) contained 1 mM of DMP, guaiacol and ABTS or 25–200 μM of dyes, 0.25 μg/mL (for oxidation assays) or 0.5 μg/mL (for decolorization assays) of purified enzyme. The reaction was initiated by 0.1 mM H2O2 at 35 °C, pH 3.5. Samples without enzyme served as the control