Table 2 Steady-state kinetic constants (K_m (μM), k_cat (s ⁻¹), k_cat/K_m (M⁻¹ s⁻¹)) for the oxidation of ABTS, DMP and RB 19 by Il-DyP4 and the W variants

ABTS

K_m

67.29 + 13.05

66.44 ± 3.32

63.21 ± 12.86

71.48 ± 10.52

71.87 ± 20.86

752.00 ± 207.21

k_cat

790.00 ± 56.06

602.50 ± 11.00

786.70 ± 57.42

665.10 ± 36.90

384.85 ± 42.27

316.16 ± 61.61

k_cat/K_m

(1.17 ± 0.43) × 10⁷

(9.03 ± 3.31) × 10⁶

(1.25 ± 0.45) × 10⁷

(9.30 ± 3.51) × 10⁶

(5.35 ± 2.02) × 10⁶

(4.20 ± 2.90) × 10⁵

DMP

K_m

8.98 ± 1.02

172.71 ± 38.31

69.19 ± 10.56

86.67 ± 12.98

229.05 ± 30.79

–^b

k_cat

58.99 ± 0.45

69.65 ± 3.46

72.66 ± 1.74

63.75 ± 1.62

71.21 ± 2.60

–^b

k_cat/K_m

(6.57 ± 0.44) × 10⁶

(4.03 ± 0.90) × 10⁵

(1.05 ± 0.16) × 10⁶

(7.3 ± 1.25) × 10⁵

(3.11 ± 0.84) × 10⁵

–^b

RB 19

K_m

139.87 ± 42.85

86.88 ± 12.28

97.59 ± 24.50

74.04 ± 14.38

27.60 ± 7.70

–^b

k_cat

187.90 ± 37.00

112.63 ± 10.15

148.99 ± 21.92

108.79 ± 11.16

46.11 ± 5.91

–^b

k_cat/K_m

(1.34 ± 0.86) × 10⁶

(1.30 ± 0.83) × 10⁶

(1.53 ± 0.89) × 10⁶

(1.47 ± 0.78) × 10⁶

(1.67 ± 0.76) × 10⁶

–^b