Kinetic constantsa | Il-DyP4c | W264F c | W264H c | W264R c | W264Y c | |
---|---|---|---|---|---|---|
ABTS | Km | 67.29 ± 13.05 | 71.87 ± 20.86 | 68.90 ± 14.06 | 19.47 ± 4.41 | 62.30 ± 14.99 |
kcat | 790 ± 56.06 | 384.85 ± 42.27 | 684.34 ± 52.02 | 52.78 ± 2.86 | 243.13 ± 23.59 | |
kcat/Km | (1.17 ± 0.43) × 107 | (5.35 ± 2.02) × 106 | (9.93 ± 3.70) × 106 | (2.71 ± 0.65) × 106 | (3.90 ± 1.57) × 106 | |
DMP | Km | 8.98 ± 1.02 | 229.05 ± 30.78 | 42.64 ± 6.32 | 1082.00 ± 283.00 | 953.00 ± 102.70 |
kcat | 58.99 ± 0.45 | 71.21 ± 2.60 | 54.29 ± 1.08 | 18.28 ± 2.30 | 56.25 ± 2.81 | |
kcat/Km | (6.57 ± 0.44) × 106 | (3.11 ± 0.84) × 105 | (1.27 ± 0.17) × 106 | (1.69 ± 0.81) × 104 | (5.90 ± 2.74) × 104 | |
RB19 | Km | 139.87 ± 42.85 | 27.60 ± 7.70 | 66.50 ± 25.31 | –b | –b |
kcat | 187.90 ± 37.00 | 46.11 ± 5.91 | 86.87 ± 19.20 | –b | –b | |
kcat/Km | (1.34 ± 0.86) × 106 | (1.67 ± 0.76) × 106 | (1.31 ± 0.76) × 106 | –b | –b |