Fig. 3

Soluble and insoluble COS pools expressed in the form of αGlc1-P equivalents. A Soluble (black and red bars) and insoluble COS (green and yellow bars) pools calculated from the last sample of experimental (black and green bars) or predicted time courses (red and yellow bars). Averages and corresponding standard deviations obtained from models PE18–PE20 are shown. B–E show time courses of soluble and insoluble COS obtained from experiments (black circles) and predicted by the model (lines). Experiments carried out at a αGlc1-P/G2 ratio of 4.0 (B and C) and 3.0 (D, E) served as basis. Solid lines indicate representative data predicted by PE18 while dashed lines indicate boundaries of solution spaces obtained from all three models. Of note: Predicted characteristics of COS pools over time in Panel B and C were almost identical for all three models (R² = 0.961 ± 0.0002 (B), R² = 0.965 ± 0.0002 (C)). Shown solutions spaces in D and E were obtained within ranges for CcCdP of 1.0 ± 0.05 mmol/L/min and ± 0.5 mM for both substrates