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Table 1 Boundaries applied in parameter estimation analyses

From: Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides

Parameter

LB

UB

CdP reaction model

 KiGlc1P, [mM]

0.17

0.32

 KGlc1P, [mM]

0.23

0.72

 KG2, [mM]

5.80

6.25

 KG3, [mM]

18.0

24.5

 KG4 (= KG5KG11), [mM]

1.0

100.0

 Keq

0.50

3.60

 k2, [−]

1.60

2.00

 k3, [−]

1.00

5.00

 k4, [−]

1.00

5.00

 k5 (= k6k10), [−]

1.00

5.00

Precipitation

 [G5]SL, [mM]

14.35

25.64

 [G6]SL, [mM]

2.68

4.79

 [G7]SL, [mM]

0.501

0.896

 [G8]SL, [mM]

0.0937

0.1674

 [G9]SL, [mM]

0.0175

0.0313

 [G10]SL, [mM]

0.00327

0.00585

 [G11]SL, [mM]

0.000612

0.001093

 [G12]SL, [mM]

0.000114

0.000204

Experiment

 Vmax, [mM/min]

  

 All

0.95

1.05

 [G2]t=0, [mM]

  

 αGlc1-P/G2: 0.2, 1.0

49.5

50.5

 αGlc1-P/G2: 2.0, 4.0

24.5

25.5

[αGlc1-P]t=0, [mM]

 αGlc1-P/G2: 0.2

9.5

10.5

 αGlc1-P/G2: 1.0, 2.0

49.5

50.5

 αGlc1-P/G2: 4.0

99.5

100.5

  1. LB lower boundaries, UB upper boundaries